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PXD005829-2

PXD005829 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe 3’-UTR of MYC couples RNA polymerase II function to ribonucleotide levels
DescriptionDeregulated expression of MYC enhances glutamine utilization and renders cell survival dependent on glutamine, inducing “glutamine addiction”. Surprisingly, colon cancer cells that express high levels of MYC due to WNT pathway mutations, are not glutamine-addicted but undergo a reversible cell cycle arrest upon glutamine deprivation. We show here that glutamine deprivation suppresses translation of endogenous MYC via the 3’-UTR of the MYC mRNA, enabling escape from apoptosis. This regulation is mediated by glutamine-dependent changes in adenosine nucleotide levels. Glutamine deprivation causes a global reduction in promoter association of RNA Polymerase II (RNAPII) and slows transcriptional elongation. While activation of MYC restores binding of MYC and RNAPII function on most promoters, restoration of elongation is imperfect and activation of MYC in the absence of glutamine causes stalling of RNAPII on multiple genes, correlating with R-loop formation. Stalling of RNAPII and R-loop formation can cause DNA damage, arguing that the MYC 3’-UTR is critical for maintaining genome stability when ribonucleotide levels are low.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:49:22.965.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJens Vanselow
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-02-01 06:43:54ID requested
12022-02-28 03:02:59announced
22023-11-14 08:49:23announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: myc,mRNA-protein interaction, glutamine, nanoLC-MS/MS
Contact List
Andreas Schlosser
contact affiliationProf. Dr. Andreas Schlosser Rudolf-Virchow-Zentrum für Experimentelle Biomedizin Universität Würzburg Josef-Schneider-Str. 2 97080 Würzburg Germany
contact emailandreas.schlosser@uni-wuerzburg.de
lab head
Jens Vanselow
contact affiliationUniversity Wuerzburg
contact emailjens.vanselow@uni-wuerzburg.de
dataset submitter
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Dataset FTP location
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