PXD005214 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteins in extracellular vehicles as candidate markers for breast cancer |
Description | The state of protein phosphorylation can be a key determinant of cellular physiology such as early stage cancer, but the development of phosphoproteins from biofluids for disease diagnosis remains elusive. Here we demonstrate, for the first time, a strategy to isolate and identify phosphoproteins in extracellular vehicles from human plasma as potential markers to differentiate disease from healthy states. We identified close to 10,000 unique phosphopeptides, representing 2108 phosphoproteins, in EVs isolated from small volume of plasma samples. Using label-free quantitative proteomics, we quantified 144 phosphoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer than in healthy controls. Multiple novel biomarkers were validated in 13 patients using Paralleled Reaction Monitoring and antibody- and non-antibody based assays. The study demonstrated that the development of phosphoproteins in plasma EV as disease biomarkers is highly feasible and may transform cancer screening and monitoring. |
HostingRepository | PRIDE |
AnnounceDate | 2017-03-23 |
AnnouncementXML | Submission_2017-03-23_10:15:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | I-Hsuan Chen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-10-24 03:43:35 | ID requested | |
⏵ 1 | 2017-03-23 10:15:06 | announced | |
Publication List
Chen IH, Xue L, Hsu CC, Paez JS, Pan L, Andaluz H, Wendt MK, Iliuk AB, Zhu JK, Tao WA, Phosphoproteins in extracellular vesicles as candidate markers for breast cancer. Proc Natl Acad Sci U S A, 114(12):3175-3180(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Extracellular vesicles, phosphoproteomics, plasma, biomarker |
Contact List
Weiguo Andy Tao |
contact affiliation | Department of Biochemistry, Purdue University, West Lafayette, IN USA |
contact email | taow@purdue.edu |
lab head | |
I-Hsuan Chen |
contact affiliation | Purdue University |
contact email | chen1329@purdue.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005214
- Label: PRIDE project
- Name: Phosphoproteins in extracellular vehicles as candidate markers for breast cancer