PXD005214

PXD005214 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteins in extracellular vehicles as candidate markers for breast cancer
Description	The state of protein phosphorylation can be a key determinant of cellular physiology such as early stage cancer, but the development of phosphoproteins from biofluids for disease diagnosis remains elusive. Here we demonstrate, for the first time, a strategy to isolate and identify phosphoproteins in extracellular vehicles from human plasma as potential markers to differentiate disease from healthy states. We identified close to 10,000 unique phosphopeptides, representing 2108 phosphoproteins, in EVs isolated from small volume of plasma samples. Using label-free quantitative proteomics, we quantified 144 phosphoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer than in healthy controls. Multiple novel biomarkers were validated in 13 patients using Paralleled Reaction Monitoring and antibody- and non-antibody based assays. The study demonstrated that the development of phosphoproteins in plasma EV as disease biomarkers is highly feasible and may transform cancer screening and monitoring.
HostingRepository	PRIDE
AnnounceDate	2017-03-23
AnnouncementXML	Submission_2017-03-23_10:15:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	I-Hsuan Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-24 03:43:35	ID requested
⏵ 1	2017-03-23 10:15:06	announced

Chen IH, Xue L, Hsu CC, Paez JS, Pan L, Andaluz H, Wendt MK, Iliuk AB, Zhu JK, Tao WA, Phosphoproteins in extracellular vesicles as candidate markers for breast cancer. Proc Natl Acad Sci U S A, 114(12):3175-3180(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Extracellular vesicles, phosphoproteomics, plasma, biomarker

Weiguo Andy Tao
contact affiliation	Department of Biochemistry, Purdue University, West Lafayette, IN USA
contact email	taow@purdue.edu
lab head
I-Hsuan Chen
contact affiliation	Purdue University
contact email	chen1329@purdue.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005214
     1. Label: PRIDE project
     2. Name: Phosphoproteins in extracellular vehicles as candidate markers for breast cancer