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PXD005203-1

PXD005203 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStaphylococcus aureus Secretome Profiling using Label Free Quantitation
Description The application of label free quantitation (LFQ) to the profiling of secreted proteomes is a powerful tool in the deciphering of these mechanisms and the determination of the severity of infection with different strains. Our study consists of three distinct, but interconnected experiments resulting in an extensive data set for the identification of antigens. First we explore the changes in virulence factor secretion when regulators are mutated, then compare these effects in two different growth conditions. We further explore the effects of nutrient accessibility by profiling the secreted proteomes of S. aureus at different points in the colony growth curve in both a rich and minimal media. Lastly, the secreted proteins from a selection of twelve strain types representing 5 colony complexes are quantified and compared to the cytotoxicity of the strains. Biological triplicates were used for mutant and growth curve studies.
HostingRepositoryMassIVE
AnnounceDate2017-02-14
AnnouncementXMLSubmission_2017-02-14_05:29:20.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJessica Chapman
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
Instrumentinstrument model: Fusion Lumos Tribrid with ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-10-21 07:04:17ID requested
12017-02-14 05:29:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: secretome, label free quantitation
Contact List
principal_investigator
lab head
Jessica Chapman
contact affiliationNYU School of Medicine
contact emailjessica.chapman@nyumc.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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