PXD005200-1

PXD005200 is an original dataset announced via ProteomeXchange.

Title	Comprehensive and at copy number resolution analysis of the red blood cells proteome unravels unexpected membrane transporters
Description	Red blood cells (RBC) are the most abundant cell type in human body that are responsible not only for tissue transport of oxygen and carbon dioxide, but also for a range of other functions. RBC and, in particular, their plasma membrane have been extensively studied for many years. These investigations led to composite models of RBC membrane organization. During the past decade proteomic studies extended our knowledge on RBC composition, however these studies were, at their best, able to provide only semiquantitative insights. Here we report a large scale proteomic investigation of RBCs and their ‘white ghost’ membrane fraction. Our in depth analysis resulted in identification of 2,650 proteins of which we consistently quantified 990 proteins in the RBC membrane. We quantified 41 membrane transporter proteins spanning 5 orders of magnitude dynamic range. Some of these, including the drug transporter ABCA7 and choline transporters SLC44A1 and SLC44A2 were previously not reported for the RBC membrane. Comparison of protein copy number values assessed by proteomics showed a good correlation with literature data, however abundances of few proteins were not consistent with the classical references. Since we validated our data by a targeted analysis using labelled standards we suggest to consider some older reference data from a variety of biochemical approaches as inaccurate. Our study provide the first comprehensive and quantitative analysis of the RBCs which may have implications for studying erythrocyte structure, functions and disease.
HostingRepository	PRIDE
AnnounceDate	2017-08-11
AnnouncementXML	Submission_2017-08-11_00:06:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacek Wisniewski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-20 23:48:56	ID requested
⏵ 1	2017-08-11 00:06:01	announced

Bryk AH, Wi, ś, niewski JR, Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res, 16(8):2752-2761(2017) [pubmed]

curator keyword: Biological

submitter keyword: Human red blood cell, FASP, "Total Protein Approach"

Jacek R Wisniewski
contact affiliation	Max-Planck-Institute of Biochemistry Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliation	Proteomics
contact email	jwisniew@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005200
     1. Label: PRIDE project
     2. Name: Comprehensive and at copy number resolution analysis of the red blood cells proteome unravels unexpected membrane transporters