PXD005137 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic portraits of egg quality in zebrafish (Danio rerio) |
Description | Humans and animals have problems producing eggs with high embryo developmental competence, but the causes of poor egg quality are usually unknown. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading model for vertebrate development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were used to create pooled or replicated sample sets subjected to different levels of fractionation before LC-MS/MS. Obtained spectra were searched against a custom zebrafish proteome database and detected proteins were annotated, categorized and quantified based on their normalized spectral counts. Manual and automated enrichment analyses were highly confirmative, showing that good and poor quality eggs have disparate proteomes. Proteins involved in protein synthesis, energy metabolism, and lipid metabolism, and certain vitellogenin products were strikingly underrepresented in poor quality eggs. Poor quality eggs also had significantly higher representation of proteins related to immune system and endosome/lysosome functioning, oncogenes, and apoptosis, as well as lectins and egg envelope proteins. Quantitative comparisons of highly abundant proteins revealed 9 candidate egg quality markers warranting further study. In conclusion, the zebrafish egg proteome appears to be linked to embryo developmental potential, a phenomenon that begs further investigation. |
HostingRepository | PRIDE |
AnnounceDate | 2017-11-27 |
AnnouncementXML | Submission_2017-11-27_01:55:34.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005137 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | E Com |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-10-12 05:02:21 | ID requested | |
⏵ 1 | 2017-11-27 01:55:35 | announced | |
Publication List
Yilmaz O, Patinote A, Nguyen TV, Com E, Lavigne R, Pineau C, Sullivan CV, Bobe J, Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio). PLoS One, 12(11):e0188084(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: egg quality, proteome profiles, protein markers, zebrafish |
Contact List
Charles Pineau |
contact affiliation | Protim Irset Inserm U1085 |
contact email | charles.pineau@inserm.fr |
lab head | |
E Com |
contact affiliation | Plate-Forme protéomique Biogenouest |
contact email | emmanuelle.com@univ-rennes1.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005137
- Label: PRIDE project
- Name: Proteomic portraits of egg quality in zebrafish (Danio rerio)