PXD005137

PXD005137 is an original dataset announced via ProteomeXchange.

Title	Proteomic portraits of egg quality in zebrafish (Danio rerio)
Description	Humans and animals have problems producing eggs with high embryo developmental competence, but the causes of poor egg quality are usually unknown. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading model for vertebrate development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were used to create pooled or replicated sample sets subjected to different levels of fractionation before LC-MS/MS. Obtained spectra were searched against a custom zebrafish proteome database and detected proteins were annotated, categorized and quantified based on their normalized spectral counts. Manual and automated enrichment analyses were highly confirmative, showing that good and poor quality eggs have disparate proteomes. Proteins involved in protein synthesis, energy metabolism, and lipid metabolism, and certain vitellogenin products were strikingly underrepresented in poor quality eggs. Poor quality eggs also had significantly higher representation of proteins related to immune system and endosome/lysosome functioning, oncogenes, and apoptosis, as well as lectins and egg envelope proteins. Quantitative comparisons of highly abundant proteins revealed 9 candidate egg quality markers warranting further study. In conclusion, the zebrafish egg proteome appears to be linked to embryo developmental potential, a phenomenon that begs further investigation.
HostingRepository	PRIDE
AnnounceDate	2017-11-27
AnnouncementXML	Submission_2017-11-27_01:55:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005137
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	E Com
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-12 05:02:21	ID requested
⏵ 1	2017-11-27 01:55:35	announced

Yilmaz O, Patinote A, Nguyen TV, Com E, Lavigne R, Pineau C, Sullivan CV, Bobe J, Scrambled eggs: Proteomic portraits and novel biomarkers of egg quality in zebrafish (Danio rerio). PLoS One, 12(11):e0188084(2017) [pubmed]

curator keyword: Biological

submitter keyword: egg quality, proteome profiles, protein markers, zebrafish

Charles Pineau
contact affiliation	Protim Irset Inserm U1085
contact email	charles.pineau@inserm.fr
lab head
E Com
contact affiliation	Plate-Forme protéomique Biogenouest
contact email	emmanuelle.com@univ-rennes1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD005137

PRIDE project URI

• PRIDE
  1. PXD005137
     1. Label: PRIDE project
     2. Name: Proteomic portraits of egg quality in zebrafish (Danio rerio)