PXD005031 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A quantitative map of proteome dynamics during neuronal differentiation |
Description | Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis and synapse formation. To systematically profile protein expression during neuronal differentiation we have used cultured hippocampal neurons at different developmental stages in combination with triplex stable isotope dimethyl labeling technique coupled to high-resolution tandem mass spectrometry (LC-MS/MS). In total >1,500 proteins show more than two-fold expression changes during the different developmental steps, indicating extensive remodeling of the neuron proteome during differentiation. To demonstrate the strength of our resource, we focused on neural cell adhesion molecule 1 (NCAM1) as a regulator for dendritic outgrowth during neuronal development. The transmembrane isoform of NCAM1, NCAM180, is strongly upregulated during dendrite outgrowth, highly enriched in dendritic growth cones and interacts with a large variety of actin binding proteins. Inducing actin polymerization rescues the NCAM1 knockdown phenotype, suggesting that NCAM180 stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. Thus, our quantitative map of neuronal proteome dynamics will serve as a rich resource for further analyses of neurodevelopmental regulated processes. |
HostingRepository | PRIDE |
AnnounceDate | 2017-01-17 |
AnnouncementXML | Submission_2017-01-17_03:56:47.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Frese |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-09-26 00:31:29 | ID requested | |
⏵ 1 | 2017-01-17 03:56:48 | announced | |
2 | 2017-06-09 08:54:14 | announced | Updated publication reference for PubMed record(s): 28178528. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Neuronal differentiation, neuronal development, dendrite outgrowth, adhesion molecules, NCAM1, adhesion molecule, proteome, quantitative proteomics, stable isotope dimethyl labeling, mass spectrometry |
Contact List
AF Maarten Altelaar |
contact affiliation | Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands |
contact email | m.altelaar@uu.nl |
lab head | |
Christian Frese |
contact affiliation | CECAD Research Center Cologne |
contact email | cfrese@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005031 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005031
- Label: PRIDE project
- Name: A quantitative map of proteome dynamics during neuronal differentiation