PXD004966 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Cdc14 phopho-targets in response to DNA damage |
Description | We have previously shown that the Cdc14 phosphatase is essential for an efficient repair of a DNA lesion, however we still missing how the phosphatase exerts this molecular functions and what are its targets during the repair process. To identify Cdc14 phospho-targets in response to DNA damage, we performed mass spectrometry analysis of Wild-type and Cdc14 deficient cells before and after inducing a single DSBs by expressing the HO endonuclease. Wild-type and a thermosensitive allele cdc14-1 were grown overnight and blocked in G2/M by using nocodazole to avoid cell cycle-dependent changes in protein phosphorylation between both strains. After the block was attained, cells were transfer to 37C to deplete Cdc14 activity prior HO induction. By, using this approach we have screened for proteins containing quantitate low levels of phosphorylated residues after the induction of the DNA lesion that occurs only when Cdc14 is active. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_03:14:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Faull |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-09-14 02:24:00 | ID requested | |
1 | 2016-12-22 04:10:17 | announced | |
⏵ 2 | 2017-10-24 03:14:45 | announced | Updated project metadata. |
Publication List
Villoria MT, Ramos F, Due, ñ, as E, Faull P, Cutillas PR, Clemente-Blanco A, Stabilization of the metaphase spindle by Cdc14 is required for recombinational DNA repair. EMBO J, 36(1):79-101(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Saccharomyces cerevisiae, Cdc14, DNA damage |
Contact List
Peter Allen Faull |
contact affiliation | MRC Clinical Sciences Centre Proteomics and Mass Spectrometry Facility |
contact email | peter.faull@csc.mrc.ac.uk |
lab head | |
Peter Faull |
contact affiliation | MRC Clinical Sciences Centre |
contact email | peter.faull@csc.mrc.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004966 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004966
- Label: PRIDE project
- Name: Identification of Cdc14 phopho-targets in response to DNA damage