PXD004966

PXD004966 is an original dataset announced via ProteomeXchange.

Title	Identification of Cdc14 phopho-targets in response to DNA damage
Description	We have previously shown that the Cdc14 phosphatase is essential for an efficient repair of a DNA lesion, however we still missing how the phosphatase exerts this molecular functions and what are its targets during the repair process. To identify Cdc14 phospho-targets in response to DNA damage, we performed mass spectrometry analysis of Wild-type and Cdc14 deficient cells before and after inducing a single DSBs by expressing the HO endonuclease. Wild-type and a thermosensitive allele cdc14-1 were grown overnight and blocked in G2/M by using nocodazole to avoid cell cycle-dependent changes in protein phosphorylation between both strains. After the block was attained, cells were transfer to 37C to deplete Cdc14 activity prior HO induction. By, using this approach we have screened for proteins containing quantitate low levels of phosphorylated residues after the induction of the DNA lesion that occurs only when Cdc14 is active.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:14:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Faull
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-14 02:24:00	ID requested
1	2016-12-22 04:10:17	announced
⏵ 2	2017-10-24 03:14:45	announced	Updated project metadata.

Villoria MT, Ramos F, Due, ñ, as E, Faull P, Cutillas PR, Clemente-Blanco A, Stabilization of the metaphase spindle by Cdc14 is required for recombinational DNA repair. EMBO J, 36(1):79-101(2017) [pubmed]

curator keyword: Biological

submitter keyword: Saccharomyces cerevisiae, Cdc14, DNA damage

Peter Allen Faull
contact affiliation	MRC Clinical Sciences Centre Proteomics and Mass Spectrometry Facility
contact email	peter.faull@csc.mrc.ac.uk
lab head
Peter Faull
contact affiliation	MRC Clinical Sciences Centre
contact email	peter.faull@csc.mrc.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004966

PRIDE project URI

• PRIDE
  1. PXD004966
     1. Label: PRIDE project
     2. Name: Identification of Cdc14 phopho-targets in response to DNA damage