PXD004867-1

PXD004867 is an original dataset announced via ProteomeXchange.

Title	The human amyloid beta peptide interactome
Description	The amyloid beta (Aβ) peptide represents a 37 to 49 amino acids endoproteolytic fragment of the amyloid precursor protein. The cellular biology that governs the formation and clearance of Aβ has been understood to play a critical role in Alzheimer’s disease (AD). Here we identified by quantitative mass spectrometry proteins in human brain extract that bind to oligomeric Aβ1-42 (oAβ1-42) and/or monomeric Aβ1-42 (mAβ1-42) baits. Whereas several studies are available that interrogated the APP interactome by affinity purification MS, a similar investigation has, to our knowledge, not yet been reported for the Aβ peptide. To address this shortcoming, we have now undertaken an in-depth search for proteins that can bind to Aβ using biotinylated Aβ peptides as baits and human frontal lobe extract as the biological source for the capture of Aβ binding proteins. The study made use of isobaric tagging for the relative quantitation of proteins.
HostingRepository	PRIDE
AnnounceDate	2017-07-05
AnnouncementXML	Submission_2017-07-05_09:10:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Declan Williams
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-pyridylethyl-L-cysteine; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-19 01:38:41	ID requested
⏵ 1	2017-07-05 09:10:52	announced
2	2017-07-06 00:30:21	announced	Updated project metadata.
3	2017-07-19 00:05:35	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Alzheimer’s disease, amyloid beta, human, brain, frontal lobe, affinity purification, orbitrap

Gerold Schmitt-Ulms
contact affiliation	University of Toronto
contact email	g.schmittulms@utoronto.ca
lab head
Declan Williams
contact affiliation	University of Toronto
contact email	gwillym.williams@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD004867

PRIDE project URI

• PRIDE
  1. PXD004867
     1. Label: PRIDE project
     2. Name: The human amyloid beta peptide interactome