PXD004668 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interactome of Nematostella GW182 in HEK293 cells |
Description | Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria. Despite this remarkable mechanistic conservation Argonaute and GW182 differentially co-evolved in bilaterians and cnidarians seemingly leading to distinct interaction surfaces. |
HostingRepository | PRIDE |
AnnounceDate | 2016-09-27 |
AnnouncementXML | Submission_2016-09-26_21:30:18.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marieluise Kirchner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-28 02:11:38 | ID requested | |
⏵ 1 | 2016-09-26 21:30:20 | announced | |
Publication List
Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M, Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution. Nucleic Acids Res, 45(2):938-950(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: GW182, HEK293, AP-MS, LC-MSMS |
Contact List
Matthias Selbach |
contact affiliation | Proteome Dynamics Group, Max Delbrueck Center, Berlin, Germany |
contact email | matthias.selbach@mdc-berlin.de |
lab head | |
Marieluise Kirchner |
contact affiliation | Max Delbrueck Center, Berlin |
contact email | marieluise.kirchner@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004668 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004668
- Label: PRIDE project
- Name: Interactome of Nematostella GW182 in HEK293 cells