PXD004668

PXD004668 is an original dataset announced via ProteomeXchange.

Title	Interactome of Nematostella GW182 in HEK293 cells
Description	Here, we dissect the function of GW182 protein in the cnidarian Nematostella, separated by 600 million years from other Metazoa. Using cultured human cells, we show that Nematostella GW182 recruits the CCR4-NOT deadenylation complexes via its tryptophan-containing motifs, thereby inhibiting translation and promoting mRNA decay. Further, similarly to bilaterians, GW182 in Nematostella is recruited to the miRNA repression complex via interaction with Argonaute proteins, and functions downstream to repress mRNA. Thus, our work suggests that this mechanism of miRNA-mediated silencing was already active in the last common ancestor of Cnidaria and Bilateria. Despite this remarkable mechanistic conservation Argonaute and GW182 differentially co-evolved in bilaterians and cnidarians seemingly leading to distinct interaction surfaces.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-26_21:30:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marieluise Kirchner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-28 02:11:38	ID requested
⏵ 1	2016-09-26 21:30:20	announced

Mauri M, Kirchner M, Aharoni R, Ciolli Mattioli C, van den Bruck D, Gutkovitch N, Modepalli V, Selbach M, Moran Y, Chekulaeva M, Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution. Nucleic Acids Res, 45(2):938-950(2017) [pubmed]

curator keyword: Biological

submitter keyword: GW182, HEK293, AP-MS, LC-MSMS

Matthias Selbach
contact affiliation	Proteome Dynamics Group, Max Delbrueck Center, Berlin, Germany
contact email	matthias.selbach@mdc-berlin.de
lab head
Marieluise Kirchner
contact affiliation	Max Delbrueck Center, Berlin
contact email	marieluise.kirchner@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004668

PRIDE project URI

• PRIDE
  1. PXD004668
     1. Label: PRIDE project
     2. Name: Interactome of Nematostella GW182 in HEK293 cells