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PXD004615-3

PXD004615 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman iPS cell_201B7-P32
DescriptionProteome analyses of human induced pluripotent stem cell line (201B7-P32) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
HostingRepositoryjPOST
AnnounceDate2016-07-21
AnnouncementXMLSubmission_2017-03-30_23:26:15.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichio Funahashi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-20 19:45:16ID requested
12016-07-20 20:46:46announced
22016-11-17 18:04:54announcedUpdated description.
32017-03-30 23:26:16announcedUpdated Repository support.
42017-03-30 23:57:38announcedUpdated DigitalObjectIdentifier.
52018-09-18 22:27:57announcedUpdated CV, FTP location and Modification list.
62021-02-04 16:36:09announced2021-02-04: Updated FTP location.
72022-09-18 03:39:34announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: shotgun proteomics, monolithic silica column, iPS cell, one-shot proteomics
Contact List
Yasushi Ishihama
lab head
Michio Funahashi
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI