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PXD004590-1

PXD004590 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterisation of the human hemostatic O-glycoproteome
DescriptionThe hemostatic system comprises platelet aggregation, coagulation and fibrinolysis, and is critical to the maintenance of vascular integrity. Multiple studies indicate that glycans play an important role in maintaining the hemostatic system, however, most investigations have focused on N-glycans and little is known about the location and function of O-glycans. Here we performed the first systematic analysis of hemostatic O-glycosylation using lectin affinity chromatography coupled to LC-MS/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells. We identified the hitherto largest O-glycoproteome from native tissue, demonstrating that O-glycosylation is a ubiquitous modification of hemostatic proteins.
HostingRepositoryPRIDE
AnnounceDate2017-03-15
AnnouncementXMLSubmission_2017-03-15_06:01:59.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSarah King
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; Hex-HexNAc; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-18 02:51:31ID requested
12017-03-15 06:02:00announced
22017-08-06 23:57:28announcedUpdated project metadata.
32023-11-14 08:49:10announced2023-11-14: Updated project metadata.
Publication List
https://doi.org/10.1182/bloodadvances.2016002121;
Keyword List
curator keyword: Biomedical
submitter keyword: human, plasma, serum, platelet, endothelial, endothelium, hemostatic, LC-MSMS, orbitrap
Contact List
Hans Wandall
contact affiliationUniversity of Copenhagen Faculty Of Health and Medical Sciences Centre for Glycomics Department of Cellular and Molecular Medicine Building 24.6.38 Nørre Alle 20, DK-2200 Copenhagen N Denmark
contact emailhhw@sund.ku.dk
lab head
Sarah King
contact affiliationCopenhagen University
contact emailsarahki@sund.ku.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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