PXD004083

PXD004083 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Toxoplasma gondii Methylscan
Description	Arginine methylation is a common posttranslational modification found on nuclear and cytoplasmic proteins that has roles in transcriptional regulation, RNA metaboolism and DNA repair. The protozoan parasite Toxoplasma gondii has a complex life cycle requiring transcriptional plasticity and has unique transcriptional regulatory pathways. Arginine methylation probably plays an important part in transcriptional regulation and splicing biology in this organism. The T. gondii genome contains five putative protein arginine methyltransferases (PRMTs), of which PRMT1 is important for cell division and growth. In order to better understand the functions of the post translational modifiction monomethyl arginine (MMA) in T. gondii we performed a proteomic analysis of monomethyl arginine (MMA) proteins in wild type and PRMT1 knockout parasites using affinity purification employing anti-MMA specific antibodies followed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2017-02-03
AnnouncementXML	Submission_2017-02-03_01:15:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004083
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rama Yakubu
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	Methyl; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-02 02:55:41	ID requested
⏵ 1	2017-02-03 01:15:53	announced

Publication List

Yakubu RR, Silmon de Monerri NC, Nieves E, Kim K, Weiss LM, . Mol Cell Proteomics, 16(4):567-580(2017) [pubmed]

Yakubu RR, Silmon de Monerri NC, Nieves E, Kim K, Weiss LM, . Mol Cell Proteomics, 16(4):567-580(2017) [pubmed]

Keyword List

submitter keyword: Toxoplasma gondii, Methylation, Post-translational Modification, Cell Cycle, Transcription Regulation, Pathogen, Parasite, Immunoaffinity, Microbiology

submitter keyword: Toxoplasma gondii, Methylation, Post-translational Modification, Cell Cycle, Transcription Regulation, Pathogen, Parasite, Immunoaffinity, Microbiology

Contact List

Louis M. Weiss
contact affiliation	Albert Einstein College of Medicine
contact email	louis.weiss@einstein.yu.edu
lab head
Rama Yakubu
contact affiliation	Albert Einstein College of Medicine
contact email	ramarasheed.yakubu@med.einstein.yu.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD004083
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD004083

PRIDE project URI

Repository Record List

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