PXD004036 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Antimicrobial resistance of Staphylococcus aureus by quantitative proteomics with TMT-labelling |
Description | The clinical development of antimicrobial peptides (AMPs) is currently under evaluation to combat the rapid increase in multi-drug resistant bacterial pathogens. However, many AMPs closely resemble components of the human innate immune system, and the ramifications of prolonged bacterial exposure to AMPs are not fully understood. Here we show that in vitro serial passage of a clinical USA300 methicillin-resistant Staphylococcus aureus strain in a host-mimicking environment containing host-derived AMPs results in the selection of stable AMP-resistance. AMP-resistant S. aureus mutants often displayed little to no fitness cost and caused invasive disease in mice. Further, this phenotype coincided with diminished susceptibility to both clinically prescribed antibiotics and human defense peptides. These findings suggest that therapeutic use of AMPs could select for virulent mutants with cross-resistance to human innate immunity as well as antibiotic therapy. Thus, therapeutic use of AMPs and the implications of cross-resistance need to be carefully monitored and evaluated. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_01:43:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Egor Vorontsov |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-22 06:41:25 | ID requested | |
1 | 2016-09-26 07:38:47 | announced | |
⏵ 2 | 2017-10-24 01:43:39 | announced | Updated project metadata. |
Publication List
Kubicek-Sutherland JZ, Lofton H, Vestergaard M, Hjort K, Ingmer H, Andersson DI, Antimicrobial peptide exposure selects for Staphylococcus aureus resistance to human defence peptides. J Antimicrob Chemother, 72(1):115-127(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: TMT, Orbitrap Fusion, high-pH reverse-phase fractionation, Staphylococcus aureus, antimicobial resistance, antimicrobial peptide |
Contact List
Dan I. Andersson |
contact affiliation | Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, 75123, Uppsala, Sweden |
contact email | dan.andersson@imbim.uu.se |
lab head | |
Egor Vorontsov |
contact affiliation | Institut Pasteur |
contact email | yegor.msu@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004036
- Label: PRIDE project
- Name: Antimicrobial resistance of Staphylococcus aureus by quantitative proteomics with TMT-labelling