PXD004036

PXD004036 is an original dataset announced via ProteomeXchange.

Title	Antimicrobial resistance of Staphylococcus aureus by quantitative proteomics with TMT-labelling
Description	The clinical development of antimicrobial peptides (AMPs) is currently under evaluation to combat the rapid increase in multi-drug resistant bacterial pathogens. However, many AMPs closely resemble components of the human innate immune system, and the ramifications of prolonged bacterial exposure to AMPs are not fully understood. Here we show that in vitro serial passage of a clinical USA300 methicillin-resistant Staphylococcus aureus strain in a host-mimicking environment containing host-derived AMPs results in the selection of stable AMP-resistance. AMP-resistant S. aureus mutants often displayed little to no fitness cost and caused invasive disease in mice. Further, this phenotype coincided with diminished susceptibility to both clinically prescribed antibiotics and human defense peptides. These findings suggest that therapeutic use of AMPs could select for virulent mutants with cross-resistance to human innate immunity as well as antibiotic therapy. Thus, therapeutic use of AMPs and the implications of cross-resistance need to be carefully monitored and evaluated.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_01:43:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Egor Vorontsov
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-22 06:41:25	ID requested
1	2016-09-26 07:38:47	announced
⏵ 2	2017-10-24 01:43:39	announced	Updated project metadata.

Kubicek-Sutherland JZ, Lofton H, Vestergaard M, Hjort K, Ingmer H, Andersson DI, Antimicrobial peptide exposure selects for Staphylococcus aureus resistance to human defence peptides. J Antimicrob Chemother, 72(1):115-127(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: TMT, Orbitrap Fusion, high-pH reverse-phase fractionation, Staphylococcus aureus, antimicobial resistance, antimicrobial peptide

Dan I. Andersson
contact affiliation	Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, 75123, Uppsala, Sweden
contact email	dan.andersson@imbim.uu.se
lab head
Egor Vorontsov
contact affiliation	Institut Pasteur
contact email	yegor.msu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004036

PRIDE project URI

• PRIDE
  1. PXD004036
     1. Label: PRIDE project
     2. Name: Antimicrobial resistance of Staphylococcus aureus by quantitative proteomics with TMT-labelling