PXD003928 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structure and proteomics on centrosome from differentiated and quiescent cells: the thymocyte case |
Description | We have applied cryo-tomography and proteomics analyses to centrosomes isolated from young lamb thymus– a convenient source of quiescent and differentiated cells. Those analyses have allow us to better depict the protein organization joining the two centrioles in such a cellular system, in the form of a disperse network of fibers and an amorphous density, both parts linked together. Protein composition of the centrosome have been compared with published data on centrosome extracted from cell culture , and presence of various protein reported implicated in the disengagement and dissociation processes questioned. C-NAP1, cohesin smc1 and furthermore condensin smc4 and ncapd2 have been localized on the amorphous density, however DNA hasn't. Subtomogram averaging processing have been applied to the centriolar wall, both on the proximal microtubule triplets and distal doublets; results have been compared to the already published structures of centriolar wall triplet from distant species basal bodies. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-08 |
AnnouncementXML | Submission_2019-11-08_09:03:00.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003928 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Adán Alpízar |
SpeciesList | scientific name: Ovis aries (Sheep); NCBI TaxID: 9940; |
ModificationList | monohydroxylated residue; acetylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-08 03:17:19 | ID requested | |
⏵ 1 | 2019-11-08 09:03:01 | announced | |
Publication List
Busselez J, Chich, ó, n FJ, Rodr, í, guez MJ, Alp, í, zar A, Gharbi SI, Franch M, Melero R, Paradela A, Carrascosa JL, Carazo JM, Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes. Sci Rep, 9(1):7187(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Centrosome, Proteomics, Cryo-tomography, Centriolar disengagement, Cohesin |
Contact List
Jose Maria Carazo |
contact affiliation | Estructura de Macromoléculas (CNB-CSIC) |
contact email | carazo@cnb.csic.es |
lab head | |
Adán Alpízar |
contact affiliation | CNB |
contact email | adan.alpizar@cnb.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003928
- Label: PRIDE project
- Name: Structure and proteomics on centrosome from differentiated and quiescent cells: the thymocyte case