PXD003927 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Absolute Quantification of 10 Centrosomal Proteins |
Description | Centrioles are essential for the formation of centrosomes and cilia. While numerical and/or structural centrosomes aberrations are implicated in cancer, mutations in centriolar and centrosomal proteins are genetically linked to ciliopathies, microcephaly and dwarfism. The evolutionarily conserved mechanisms underlying centrosome biogenesis are centered on a set of key proteins, including Plk4, Sas-6 and STIL, whose exact levels are critical to ensure accurate reproduction of centrioles during cell cycle progression. However, neither the intracellular levels of centrosomal proteins nor their stoichiometry within centrosomes are presently known. Here we have used two complementary approaches, targeted proteomics and EGFP-tagging of centrosomal proteins at endogenous loci, to measure protein abundance in cultured human cells and purified centrosomes. Our results provide a first assessment of the absolute and relative amounts of major components of the human centrosome. This quantitative information makes several predictions that will guide future mechanistic and structural studies on the assembly and function of this important organelle. |
HostingRepository | PRIDE |
AnnounceDate | 2016-07-22 |
AnnouncementXML | Submission_2016-07-22_08:40:58.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003927 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Schmidt |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Acetyl; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-07 12:43:24 | ID requested | |
⏵ 1 | 2016-07-22 08:40:59 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Mass spectrometry, SRM, Absolute Quantification, Centrosome |
Contact List
Alexander Schmidt |
contact affiliation | Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland |
contact email | alex.schmidt@unibas.ch |
lab head | |
Alexander Schmidt |
contact affiliation | Proteomics Core Facility |
contact email | alex.schmidt@unibas.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003927
- Label: PRIDE project
- Name: Absolute Quantification of 10 Centrosomal Proteins