PXD003927

PXD003927 is an original dataset announced via ProteomeXchange.

Title	Absolute Quantification of 10 Centrosomal Proteins
Description	Centrioles are essential for the formation of centrosomes and cilia. While numerical and/or structural centrosomes aberrations are implicated in cancer, mutations in centriolar and centrosomal proteins are genetically linked to ciliopathies, microcephaly and dwarfism. The evolutionarily conserved mechanisms underlying centrosome biogenesis are centered on a set of key proteins, including Plk4, Sas-6 and STIL, whose exact levels are critical to ensure accurate reproduction of centrioles during cell cycle progression. However, neither the intracellular levels of centrosomal proteins nor their stoichiometry within centrosomes are presently known. Here we have used two complementary approaches, targeted proteomics and EGFP-tagging of centrosomal proteins at endogenous loci, to measure protein abundance in cultured human cells and purified centrosomes. Our results provide a first assessment of the absolute and relative amounts of major components of the human centrosome. This quantitative information makes several predictions that will guide future mechanistic and structural studies on the assembly and function of this important organelle.
HostingRepository	PRIDE
AnnounceDate	2016-07-22
AnnouncementXML	Submission_2016-07-22_08:40:58.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003927
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-07 12:43:24	ID requested
⏵ 1	2016-07-22 08:40:59	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Mass spectrometry, SRM, Absolute Quantification, Centrosome

Alexander Schmidt
contact affiliation	Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003927
     1. Label: PRIDE project
     2. Name: Absolute Quantification of 10 Centrosomal Proteins