PXD003888 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Proteins Sensitive to Thermal Stress in Human HEK293 cells |
Description | Heat-shock is an acute insult to the mammalian proteome. The sudden elevation in temperature has far-reaching effects on protein metabolism, leads to a rapid inhibition of most protein synthesis, and the induction of protein chaperones. Using heat-shock in human HEK293 cells, in conjunction with detergent extraction and sedimentation followed by LC-MS/MS proteomic approaches, we identify proteins that lose solubility upon heat-shock. We assume that these are metastable in protein homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-14 |
AnnouncementXML | Submission_2016-04-14_00:30:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guilian Xu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | QSTAR |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-01 02:43:19 | ID requested | |
1 | 2016-04-11 08:27:37 | announced | |
⏵ 2 | 2016-04-14 00:30:44 | announced | Updated project metadata. |
Publication List
Xu G, Pattamatta A, Hildago R, Pace MC, Brown H, Borchelt DR, Vulnerability of newly synthesized proteins to proteostasis stress. J Cell Sci, 129(9):1892-901(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: HEK293, SH-SY5Y, CCF-STTG1, heat shock, detergent insoluble, LC-MS/MS |
Contact List
David R. Borchelt |
contact affiliation | Department of Neuroscience, University of Florida |
contact email | drb1@ufl.edu |
lab head | |
Guilian Xu |
contact affiliation | Neuroscience |
contact email | xugl@ufl.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003888 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003888
- Label: PRIDE project
- Name: Identification of Proteins Sensitive to Thermal Stress in Human HEK293 cells