PXD003888

PXD003888 is an original dataset announced via ProteomeXchange.

Title	Identification of Proteins Sensitive to Thermal Stress in Human HEK293 cells
Description	Heat-shock is an acute insult to the mammalian proteome. The sudden elevation in temperature has far-reaching effects on protein metabolism, leads to a rapid inhibition of most protein synthesis, and the induction of protein chaperones. Using heat-shock in human HEK293 cells, in conjunction with detergent extraction and sedimentation followed by LC-MS/MS proteomic approaches, we identify proteins that lose solubility upon heat-shock. We assume that these are metastable in protein homeostasis.
HostingRepository	PRIDE
AnnounceDate	2016-04-14
AnnouncementXML	Submission_2016-04-14_00:30:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guilian Xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-01 02:43:19	ID requested
1	2016-04-11 08:27:37	announced
⏵ 2	2016-04-14 00:30:44	announced	Updated project metadata.

Xu G, Pattamatta A, Hildago R, Pace MC, Brown H, Borchelt DR, Vulnerability of newly synthesized proteins to proteostasis stress. J Cell Sci, 129(9):1892-901(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: HEK293, SH-SY5Y, CCF-STTG1, heat shock, detergent insoluble, LC-MS/MS

David R. Borchelt
contact affiliation	Department of Neuroscience, University of Florida
contact email	drb1@ufl.edu
lab head
Guilian Xu
contact affiliation	Neuroscience
contact email	xugl@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD003888

PRIDE project URI

• PRIDE
  1. PXD003888
     1. Label: PRIDE project
     2. Name: Identification of Proteins Sensitive to Thermal Stress in Human HEK293 cells