PXD003816 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | 7-Ketocholesterol overcomes drug resistance in Chronic Myeloid Leukemia cell lines beyond MDR1 mechanism_part1 |
Description | Chronic myeloid leukemia (CML) is a myeloproliferative disease with a characteristic BCR-ABL tyrosine kinase (TK) fusion protein. Despite the clinical efficacy accomplished by TKIs therapies disease progression may affect patient response rate to these inhibitors due to a multitude of factors that could lead to development of a mechanism known as multidrug resistance (MDR). 7-ketocholesterol (7KC) has been extensively reported to cause cell death in a variety of cancer models. In this study, we showed the in vitro efficacy of 7KC against MDR leukemia cell line, Lucena. 7KC treatment induced reduction in cell viability, together with apoptosis-mediated cell death. Moreover, downregulation of MDR protein caused intracellular drug accumulation and 7KC co-incubation with either Daunorubicin or Vincristine reduced cell viability compared to the use of each drug alone. Additionally, quantitative label-free mass spectrometry-based protein quantification showed alteration of different molecular pathways involved in cell cycle arrest, induction of apoptosis and misfolded protein response. Conclusively, this study highlights the effect of 7KC as a sensitizing agent of multidrug resistance CML and elucidate its molecular mechanisms. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:55.128.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Giuseppe Palmisano |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-03-18 06:52:32 | ID requested | |
1 | 2022-05-30 06:53:14 | announced | |
⏵ 2 | 2023-11-14 08:48:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: multidrug resistance, cell death and 7-ketocholesterol., chronic myeloid leukemia, quantitative proteomics,human, Oxysterols |
Contact List
Giuseppe Palmisano |
contact affiliation | GlycoProteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, Avenida Lineu Prestes 1374, CEP 05508-000, Sao Paulo, Brazil |
contact email | palmisano.gp@gmail.com |
lab head | |
Giuseppe Palmisano |
contact affiliation | University of Sao Paulo |
contact email | palmisano.gp@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003816
- Label: PRIDE project
- Name: 7-Ketocholesterol overcomes drug resistance in Chronic Myeloid Leukemia cell lines beyond MDR1 mechanism_part1