PXD003685 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The adaptive metabolic response involves specific protein glutathionylation during the filamentation process in the pathogen Candida albicans |
Description | Candida albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to the systemic disease candidiasis. Its ability to adopt various morphological forms, such as unicellular yeasts, filamentous pseudohyphae and hyphae, contributes to its ability to survive within the host. It has been suggested that the antioxidant glutathione is involved in the filamentation process. We investigated S-glutathionylation, the reversible binding of glutathione to proteins, and the functional consequences on C. albicans metabolic remodeling during the yeast-to-hyphae transition. Our work provided evidence for the specific glutathionylation of mitochondrial proteins involved in bioenergetics pathways in filamentous forms and a regulation of the main enzyme of the glyoxylate cycle, isocitrate lyase, by glutathionylation. Our proteomic data demonstrate that the binding of one glutathione molecule to isocitrate lyase in the hyphal forms leads to enzyme inactivation which was reversed by glutaredoxin treatment. We also assessed the effect of alternative carbon sources on glutathione levels and isocitrate lyase activity. Changes in nutrient availability led to morphological flexibility and was related to perturbations in glutathione levels and isocitrate lyase activity, confirming the key role of the maintenance of intracellular redox status in the adaptive metabolic strategy of the pathogen. |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-21 |
AnnouncementXML | Submission_2016-04-21_00:15:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Camille GARCIA |
SpeciesList | scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue; L-cysteine glutathione disulfide |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-25 01:22:52 | ID requested | |
⏵ 1 | 2016-04-21 00:15:33 | announced | |
Publication List
Gergondey R, Garcia C, Serre V, Camadro JM, Auch, รจ, re F, The adaptive metabolic response involves specific protein glutathionylation during the filamentation process in the pathogen Candida albicans. Biochim Biophys Acta, 1862(7):1309-23(2016) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Candida albicans / filamentation/ glutathionylation/ isocitrate lyase / mitochondria |
Contact List
Jean-Michel Camadro |
contact affiliation | Institut Jacques Monod |
contact email | jean-michel.camadro@ijm.fr |
lab head | |
Camille GARCIA |
contact affiliation | Institut Jacques Monod |
contact email | camille.garcia@ijm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003685
- Label: PRIDE project
- Name: The adaptive metabolic response involves specific protein glutathionylation during the filamentation process in the pathogen Candida albicans