<<< Full experiment listing

PXD003679-1

PXD003679 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReduced-representation Phosphosignatures Measured by Quantitative Targeted MS
DescriptionAbelin JG, Patel J, Lu X, Feeney CM, Fagbami L, Creech AL, Hu R, Lam D, Davison D, Pino L, Qiao JW, Kuhn E, Officer A,Li J, Abbatiello S, Subramanian A, Sidman R, Snyder E, Carr SA, Jaffe JD. Mol Cell Proteomics, 2016. Profiling posttranslational modifications represents an alternative dimension to gene expression data in characterizing cellular processes, as genetic processes alone are not sufficient to explain the entirety of biochemical mechanisms or disease etiology. For example, some cellular phenotypes resulting from chemical perturbations are partially or entirely mediated by changes in cell signaling through protein phosphorylation. To access this dimension of cellular information, we sought to develop a common platform on which cellular phosphosignaling responses could be profiled across thousands of samples. To this end, we developed a targeted MS assay that profiles a reduced-representation set of phosphopeptides that we show to be strong indicators of cellular responses to chemical perturbagens.
HostingRepositoryMassIVE
AnnounceDate2016-04-12
AnnouncementXMLSubmission_2016-04-12_07:11:59.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarl Clauser
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListunknown modification: SILAC-R-0,6,10-K-0,4,8; Phospho; unknown modification: C-carbamidomethyl; unknown modification: M-oxidation
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-23 11:42:03ID requested
12016-04-12 07:12:00announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SILAC, Targeted MS, phosphosignature
Contact List
Jacob D. Jaffe
lab head
Karl Clauser
contact affiliationBroad Institute of MIT and Harvard
contact emailclauser@broad.mit.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079524