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PXD003624-1

PXD003624 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of BNC2 interactors in human LX2 cells using RIME
DescriptionThe goal of this study was to use Rapid Immunoprecipitation Mass spectrometry of Endogenous protein (RIME) assays (Mohammed et al., 2016) to identify transcriptional regulatory partners of the transcription factor BNC2 in LX2 cells. Protein complexes were immune-precipitated with an anti-BNC2 antibody (55220-1-AP, Proteintech) or control non-immune IgG (normal rabbit IgG #2729, Cell Signaling) before being subjected to MS.
HostingRepositoryPRIDE
AnnounceDate2022-08-05
AnnouncementXMLSubmission_2022-08-05_07:59:12.769.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003624
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJean-Marc STRUB
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-12 03:30:12ID requested
12022-08-05 07:59:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME)
human
LX2
hepatic stellate cells
BNC2
Contact List
Strub Jean-Marc
contact affiliationLaboratoire de Spectrométrie de Masse Bio-Organique Département des Sciences Analytiques, Institut Pluridisciplinaire Hubert Curien, UMR 7178 (CNRS-UdS) ECPM, Bat. R5-N0, 25 rue Becquerel F67087-Strasbourg-Cedex 2
contact emailjmstrub@unistra.fr
lab head
Jean-Marc STRUB
contact affiliationCNRS
contact emailjmstrub@unistra.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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