PXD003602 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein expression in tomato tissues as a function of Al3+ stress (Suping_Exp2) |
Description | The goal of this experiment is to identify the changes in protein expression induced by Al treatment with a view towards developing a molecular level understanding of Al tolerance in an important crop species. In this particular experiment the focus was on protein expression in leaf tissue. Tomato (Solanum lycopersicum ‘Micro-Tom’) plants were treated in Magnavaca’s solution (pH 4.5) supplemented with 50 µM AlK(SO4)2·12H2O providing 7.5 µM Al3+ activity over a four-month period beginning at the emergence of the first flower buds and ending when a few leaves started to turn yellow. Proteins from root, leaf, embryo and seed-coat tissues were identified using a 8-plex iTRAQ labeling strategy followed by a two-dimensional (high- and low-pH) chromatographic separation, and final generation of MS/MS spectra of tryptic peptides on an LTQ-Orbitrap Elite-mass spectrometer |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-25 |
AnnouncementXML | Submission_2016-04-25_01:03:02.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003602 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ted Thannhauser |
SpeciesList | scientific name: Solanum lycopersicum; NCBI TaxID: 4081; |
ModificationList | Methylthio; iTRAQ8plex; Oxidation |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-02-09 07:27:28 | ID requested | |
⏵ 1 | 2016-04-25 01:03:04 | announced | |
Publication List
Zhou S, Okekeogbu I, Sangireddy S, Ye Z, Li H, Bhatti S, Hui D, McDonald DW, Yang Y, Giri S, Howe KJ, Fish T, Thannhauser TW, Proteome Modification in Tomato Plants upon Long-Term Aluminum Treatment. J Proteome Res, 15(5):1670-84(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: tomato, roots, leaves, embryo, seed coat, iTRAQ |
Contact List
Ted Thannhauser |
contact affiliation | Robert W. Holley Center United States Department of Agriculture Agricultural Research Service |
contact email | tt34@cornell.edu |
lab head | |
Ted Thannhauser |
contact affiliation | USDA-ARS |
contact email | tt34@cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003602
- Label: PRIDE project
- Name: Protein expression in tomato tissues as a function of Al3+ stress (Suping_Exp2)