PXD003495 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Compensating the fitness costs of synonymous mutations |
Description | Synonymous mutations do not change the sequence of the polypeptide but they may still influence fitness. We investigated in Salmonella enterica how four synonymous mutations in the rpsT gene (encoding ribosomal protein S20) reduce fitness (i.e. growth rate) and the mechanisms by which this cost can be genetically compensated. The reduced growth rates of the synonymous mutants were correlated with reduced levels of the rpsT transcript and S20 protein. In an adaptive evolution experiment these fitness impairments could be compensated by mutations that either caused up-regulation of S20 through increased gene dosage (due to duplications), increased transcription of the rpsT gene (due to an rpoD mutation or mutations in rpsT), or increased translation from the rpsT transcript (due to rpsT mutations). We suggest that the reduced levels of S20 in the synonymous mutants result in production of a defective subpopulation of 30S subunits lacking S20 that reduce protein synthesis and bacterial growth and that the compensatory mutations restore S20 levels and the number of functional ribosomes. Our results demonstrate how specific synonymous mutations can cause substantial fitness reductions and that many different types intra- and extragenic compensatory mutations can efficiently restore fitness. Furthermore, our study highlights that also synonymous sites can be under strong selection, which may have implications for the use of dN/dS ratios as signature for selection. |
HostingRepository | PRIDE |
AnnounceDate | 2016-03-11 |
AnnouncementXML | Submission_2016-03-11_08:33:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Carina Sihlbom |
SpeciesList | scientific name: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720); NCBI TaxID: 99287; |
ModificationList | monohydroxylated residue; methylthiolated residue; TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-01-21 01:25:44 | ID requested | |
⏵ 1 | 2016-03-11 08:33:32 | announced | |
Publication List
Kn, ö, ppel A, N, ä, svall J, Andersson DI, Compensating the Fitness Costs of Synonymous Mutations. Mol Biol Evol, 33(6):1461-77(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Salmonella mutations, rpsT gene, ribosomal protein S20 |
Contact List
Carina Sihlbom |
contact affiliation | Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg |
contact email | carina.sihlbom@gu.se |
lab head | |
Carina Sihlbom |
contact affiliation | Proteomics Core Facility / University of Gothenburg |
contact email | carina.sihlbom@gu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003495
- Label: PRIDE project
- Name: Compensating the fitness costs of synonymous mutations