PXD003466 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of proteins stably associated with G9a/GLP (EHMT2/EHMT1) complex of histone methyltransferases |
Description | G9a (EHMT2) and the G9a-like protein GLP (EHMT1) form a stable G9a/GLP heterodimer in embryonic stem cells and function cooperatively to establish and maintain the abundant repressive H3K9me2 modification, in addition to modifying several non-histone proteins. The G9a-dependent H3K9me2 is implicated in lineage-specific gene silencing and covers large chromosomal domains. While the mechanism of H3K9me2maintenance by G9a/GLP is known, how new patterns of this modification are established is not well understood. With this in mind, we used FLAG affinity purification of G9a under two different stringency conditions (150 and 300 mM NaCl) coupled with mass spectrometry to identify proteins stably associated with G9a/GLP, which could serve as potential recruiters of the complex to unmodified chromatin. |
HostingRepository | PRIDE |
AnnounceDate | 2016-03-16 |
AnnouncementXML | Submission_2016-03-16_07:04:04.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD003466 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Irina Stancheva |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-01-15 03:11:02 | ID requested | |
⏵ 1 | 2016-03-16 07:04:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: Histones, G9a/GLP (EHMT2/EHMT1) complex, chromatin, embryonic stem cells, mouse, shotgun proteomics |
Contact List
Dr.Irina Stancheva |
contact affiliation | Wellcome Trust Centre for Cell Biology University of Edinburgh UK |
contact email | istancheva@ed.ac.uk |
lab head | |
Irina Stancheva |
contact affiliation | University of Edinburgh |
contact email | istancheva@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD003466 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003466
- Label: PRIDE project
- Name: Identification of proteins stably associated with G9a/GLP (EHMT2/EHMT1) complex of histone methyltransferases