<<< Full experiment listing

PXD003466

PXD003466 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of proteins stably associated with G9a/GLP (EHMT2/EHMT1) complex of histone methyltransferases
DescriptionG9a (EHMT2) and the G9a-like protein GLP (EHMT1) form a stable G9a/GLP heterodimer in embryonic stem cells and function cooperatively to establish and maintain the abundant repressive H3K9me2 modification, in addition to modifying several non-histone proteins. The G9a-dependent H3K9me2 is implicated in lineage-specific gene silencing and covers large chromosomal domains. While the mechanism of H3K9me2maintenance by G9a/GLP is known, how new patterns of this modification are established is not well understood. With this in mind, we used FLAG affinity purification of G9a under two different stringency conditions (150 and 300 mM NaCl) coupled with mass spectrometry to identify proteins stably associated with G9a/GLP, which could serve as potential recruiters of the complex to unmodified chromatin.
HostingRepositoryPRIDE
AnnounceDate2016-03-16
AnnouncementXMLSubmission_2016-03-16_07:04:04.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003466
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterIrina Stancheva
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-15 03:11:02ID requested
12016-03-16 07:04:05announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Histones, G9a/GLP (EHMT2/EHMT1) complex, chromatin, embryonic stem cells, mouse, shotgun proteomics
Contact List
Dr.Irina Stancheva
contact affiliationWellcome Trust Centre for Cell Biology University of Edinburgh UK
contact emailistancheva@ed.ac.uk
lab head
Irina Stancheva
contact affiliationUniversity of Edinburgh
contact emailistancheva@ed.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD003466
PRIDE project URI
Repository Record List
[ + ]