PXD003404

PXD003404 is an original dataset announced via ProteomeXchange.

Title	Labeo rohita (Rohu) plasma MALDI-TOF MS/MS
Description	Arsenic (As) is a toxic environmental contaminant and potential human carcinogen. Chronic intake of arsenic-contaminated water leads to arsenicosis that is a major public health problem in many parts of the world, including India and Bangladesh. Thus, the early detection of arsenic toxicity will greatly benefit patients. However, the detection of arsenicosis needs to be done early before onset of severe symptoms in which case the tools used for detection have to be both sensitive and reliable. In this context, the present study investigated plasma proteome changes in arsenic-exposed Labeo rohita, with the aim of identifying biomarkers for arsenicosis. Changes in the plasma proteome were investigated using gel-based proteomics technology. Using quantitative image analysis of the 2D protein profiles, unique protein spots were identified from the plasma proteome by MALDI-TOF-TOF-MS. Unique proteins identified included Apolipoprotein-A1 (Apo-A1), α-2 macroglobulin-like protein (A2ML), and transferrin. Highly up-regulated protein spots identified in plasma from arsenic-exposed fish were liver-specific, including Apo-A1, and A2ML consistent with liver damage. It is proposed that a combination of these proteins could serve as useful biomarkers of hepatotoxicity and chronic liver disease due to arsenic exposure.
HostingRepository	PRIDE
AnnounceDate	2017-05-11
AnnouncementXML	Submission_2017-05-11_01:02:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bimal Mohanty
SpeciesList	scientific name: Labeo rohita (Indian major carp); NCBI TaxID: 84645;
ModificationList	iodoacetamide derivatized residue
Instrument	AB SCIEX instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2016-01-04 07:34:27	ID requested
⏵ 1	2017-05-11 01:02:41	announced

Banerjee S, Mahanty A, Mohanty S, Mazumder DG, Cash P, Mohanty BP, Identification of potential biomarkers of hepatotoxicity by plasma proteome analysis of arsenic-exposed carp Labeo rohita. J Hazard Mater, 336():71-80(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Labeo rohita, Plasma, MALDI-TOF MS/MS

Bimal Prasanna Mohanty
contact affiliation	ICAR- Central Inland Fisheries Research Institute, Fishery Resource and Environmental Management Division, Biochemistry Laboratory, Proteomics Unit, Barrackpore- 700120. Ph- +919230618153
contact email	bimalmohanty12@rediffmail.com
lab head
Bimal Mohanty
contact affiliation	ICAR-Central Inland Fisheries Research Institute, Barrackpore
contact email	bimalmohanty12@rediffmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003404
     1. Label: PRIDE project
     2. Name: Labeo rohita (Rohu) plasma MALDI-TOF MS/MS