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PXD003392-1

PXD003392 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTagless Analysis of Protein-Protein Interactions in Desufovibrio vulgaris.
DescriptionA quantitative tagless method that employs iTRAQ mass spectrometry to measure the co-purification of endogenous proteins through orthogonal chromatographic fractionation was employed to characterize protein-protein interactions in D.vulgaris. 5,273 fractions from a four step fractionation of a D. vulgaris protein extract were assayed, leading to the detection of 1,242 proteins. Shotgun LC MALDI utilized AB Sciex 4800 and 5800 mass spectrometers. ProteinPilot software was used for protein identification and relative quantitation. Pearson cross-correlation values were computed for each iTRAQ multiplex for both the SEC and separately the HIC dimensions. Interologs of protein pairs in the established benchmark protein-protein interaction sets are much more likely to have high maximum CC values in both the HIC and SEC dimensions than seen for all protein pairs or for negative protein pairs. We identified 200 high confidence D. vulgaris PPIs based on tagless co-purification and co-localization in the genome, 140 of which are not part of our D. vulgaris affinity purification-MS interactome.
HostingRepositoryMassIVE
AnnounceDate2016-04-21
AnnouncementXMLSubmission_2016-04-21_12:41:51.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWitkowska
SpeciesList scientific name: Desulfovibrio vulgaris str. Hildenborough; NCBI TaxID: 882;
ModificationListNo PTMs are included in the dataset
Instrument4800 Proteomics Analyzer
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-01-02 13:45:50ID requested
12016-04-21 12:41:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Protein-protein interactions, tagless strategy, iTRAQ, LC MALDI TOF/TOF, Desulvofibrio
Contact List
Mark D. Biggin
lab head
Witkowska
contact affiliationUCSF
contact emailh_ewa_witkowska@yahoo.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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