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PXD003352-1

PXD003352 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.
DescriptionHere we describe a series TAP-MS analyses in various mutant backgrounds of Arabidopsis thaliana to co-purify any PCH1’s associated proteins, using tagged PCH1 as bait (PCH1-His6-Flag3, either overexpressed or under the control of its own promoter). By combining TAP-MS with genetics, we investigate the in-vivo dependence of several proteins for maintaining the association of PCH1 with its interacting proteins. Our results refine the protein-protein interaction network of an existing interactome containing phytochromes, the COP1-SPAs complex and the ELF3-ELF4-LUX complex.
HostingRepositoryPRIDE
AnnounceDate2016-02-09
AnnouncementXMLSubmission_2016-02-09_08:39:14.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD003352
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSophe Alvarez
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListOxidation; Carbamidomethyl; Deamidated
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-12-15 06:43:08ID requested
12016-02-09 08:39:15announced
Publication List
Huang H, Yoo CY, Bindbeutel R, Goldsworthy J, Tielking A, Alvarez S, Naldrett MJ, Evans BS, Chen M, Nusinow DA, PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis. Elife, 5():e13292(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Tandem AP-MS, circadian clock, Arabidopsis
Contact List
Meter Nusinow
contact affiliationDonald Danforth Plant Science Center
contact emaildnusinow@danforthcenter.org
lab head
Sophe Alvarez
contact affiliationUniversity of Nebraska - Lincoln
contact emailsalvarez@unl.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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