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PXD003344-1

PXD003344 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePlug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry
DescriptionIn this study, targeted parallel reaction monitoring assays for phosphopeptides were configured by mining data from large-scale experiments. The capabilities of the method were assessed by performing several benchmarking experiments. The accuracy of retention time prediction using a large-scale database was assessed by using BSA peptides or human phosphopeptides to predict the retention time of distinct peptides in a subsequent run. Data-driven phosphopeptide sequence and charge state was compared to heuristics-based selection using unscheduled PRM assays. Phosphoproteome analysis was performed in technical quadruplicate using parallel reaction monitoring, data-independent acquisition, and data-dependent acquisition. A label-free quantitative parallel reaction monitoring experiment was performed on phosphopeptides enriched from cells stimulated -/+ IGF-1 (n=6).
HostingRepositoryMassIVE
AnnounceDate2016-03-27
AnnouncementXMLSubmission_2016-03-27_23:43:38.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRobert Lawrence
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListPhospho
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-12-14 15:56:33ID requested
12016-03-27 23:43:39announced
Publication List
Robert T. Lawrence, Brian C. Searle, Ariadna Llovet, Judit Villén. Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry. Nature Methods. 2016 Mar. doi: 10.1038/nmeth.3811.
Keyword List
submitter keyword: phosphoproteomics, targeted analysis, PRM, DDA, DIA
Contact List
Judit Villén
lab head
Robert Lawrence
contact affiliationUniversity of Washington
contact emailroblaw@uw.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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