PXD003159 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Heptad-specific phosphorylation of RNA polymerase II CTD |
Description | The carboxy-terminal domain (CTD) of RNA polymerase II (Pol II) consists of heptad repeats with the consensus motif Y1-S2-P3-T4-S5-P6-S7. Dynamic phosphorylation of the CTD coordinates Pol II progression through the transcription cycle. Monoclonal antibodies have been used to study in vivo the potentially phosphorylated CTD amino acids (Y1, S2, T4, S5 and S7). However, the epitopes detected by antibodies can be masked by proteins or modifications at neighbouring sites. Therefore, the effectiveness of antibodies in western blot or ChIP analysis reflects the number of accessible CTD phosphorylation marks, but not the total number of phosphorylations. Most importantly, CTD phospho-specific antibodies do not provide any heptad - (location) specific information of CTD phosphorylation. Due to these limitations, the principles and patterns of CTD phosphorylation remained elusive. Here, we use genetic and mass spectrometric approaches to directly detect and map phosphosites along the entire CTD. We confirm phosphorylation of CTD residues Y1, S2, T4, S5 and S7 in mammalian and yeast cells. Although specific phosphorylation signatures dominate, adjacent CTD repeats can be differently phosphorylated, leading to a high variation of coexisting phosphosites in mono- and di-heptad CTD repeats. Inhibition of CDK9 kinase specifically reduces S2 phosphorylation levels within the CTD. |
HostingRepository | PRIDE |
AnnounceDate | 2016-01-25 |
AnnouncementXML | Submission_2016-01-25_03:11:08.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ignasi Forne |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue; phosphorylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-11-06 07:43:04 | ID requested | |
⏵ 1 | 2016-01-25 03:11:10 | announced | |
Publication List
Sch, ü, ller R, Forn, é I, Straub T, Schreieck A, Texier Y, Shah N, Decker TM, Cramer P, Imhof A, Eick D, Heptad-Specific Phosphorylation of RNA Polymerase II CTD. Mol Cell, 61(2):305-14(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Pol II CTD |
CTD heptads |
CTD phosphosites |
LC-MS/MS |
Contact List
Axel Imhof |
contact affiliation | Biomedical Center Munich Molecular Biology Großhaderner Strasse 9 82152 Planegg-Martinsried |
contact email | imhof@lmu.de |
lab head | |
Ignasi Forne |
contact affiliation | Biomedical Center-LMU |
contact email | ignasi.forne@lrz.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003159 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003159
- Label: PRIDE project
- Name: Heptad-specific phosphorylation of RNA polymerase II CTD