PXD003054 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bovine protein glycation sites in different milk products |
Description | Here we studied the glycation of bovine milk proteins by lactose as dominant sugar in milk and hexoses using tandem mass spectrometry (CID and ETD mode). In a bottom-up proteomics approach after enriching glycated peptides by boronate affinity chromatography, first we could identify 260 lactosylated peptides corresponding to 124 lactosylation sites in 28 bovine milk proteins in raw milk, raw colostrum, three brands of pasteurized milk, three brands of UHT milk, and five brands of infant formula. The same regular and additionally two lactose-free milk products (pasteurized and UHT milk) where lactose is enzymatically cleaved into the more reactive hexoses were analyzed in terms of hexosylation sites that resulted in identification of 124 hexosylated tryptic peptides corresponding to 86 glycation sites in 17 bovine milk proteins. In quantitative terms glycation increased from raw milk to pasteurized milk to UHT milk and infant formula, i.e., with the harsher processing conditions. Lactose-free milk contained significantly higher hexosylation degrees than the corresponding regular milk product. |
HostingRepository | PRIDE |
AnnounceDate | 2016-01-11 |
AnnouncementXML | Submission_2016-01-11_04:13:07.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sanja Milkovska-Stamenova |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; lactose glycated lysine; hexose glycated L-lysine |
Instrument | LTQ Orbitrap XL ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-15 00:01:02 | ID requested | |
⏵ 1 | 2016-01-11 04:13:09 | announced | |
Publication List
Milkovska-Stamenova S, Hoffmann R, Hexose-derived glycation sites in processed bovine milk. J Proteomics, 134():102-111(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Boronate affinity chromatography (BAC), nRPC-ESI-MS/MS, Electron transfer dissociation (ETD), Infant formula, Lactose-free milk, Maillard reaction, Non-enzymatic glycosylation |
Contact List
Prof. Dr. Ralf Hoffmann |
contact affiliation | Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, and Center for Biotechnology and Biomedicine, Universität Leipzig, Leipzig, Germany |
contact email | hoffmann@chemie.uni-leipzig.de |
lab head | |
Sanja Milkovska-Stamenova |
contact affiliation | Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, and Center for Biotechnology and Biomedicine, Universität Leipzig, Leipzig, Germany |
contact email | sanja.milkovska@uni-leipzig.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003054
- Label: PRIDE project
- Name: Bovine protein glycation sites in different milk products