PXD003054

PXD003054 is an original dataset announced via ProteomeXchange.

Title	Bovine protein glycation sites in different milk products
Description	Here we studied the glycation of bovine milk proteins by lactose as dominant sugar in milk and hexoses using tandem mass spectrometry (CID and ETD mode). In a bottom-up proteomics approach after enriching glycated peptides by boronate affinity chromatography, first we could identify 260 lactosylated peptides corresponding to 124 lactosylation sites in 28 bovine milk proteins in raw milk, raw colostrum, three brands of pasteurized milk, three brands of UHT milk, and five brands of infant formula. The same regular and additionally two lactose-free milk products (pasteurized and UHT milk) where lactose is enzymatically cleaved into the more reactive hexoses were analyzed in terms of hexosylation sites that resulted in identification of 124 hexosylated tryptic peptides corresponding to 86 glycation sites in 17 bovine milk proteins. In quantitative terms glycation increased from raw milk to pasteurized milk to UHT milk and infant formula, i.e., with the harsher processing conditions. Lactose-free milk contained significantly higher hexosylation degrees than the corresponding regular milk product.
HostingRepository	PRIDE
AnnounceDate	2016-01-11
AnnouncementXML	Submission_2016-01-11_04:13:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sanja Milkovska-Stamenova
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; lactose glycated lysine; hexose glycated L-lysine
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-15 00:01:02	ID requested
⏵ 1	2016-01-11 04:13:09	announced

Milkovska-Stamenova S, Hoffmann R, Hexose-derived glycation sites in processed bovine milk. J Proteomics, 134():102-111(2016) [pubmed]

curator keyword: Biological

submitter keyword: Boronate affinity chromatography (BAC), nRPC-ESI-MS/MS, Electron transfer dissociation (ETD), Infant formula, Lactose-free milk, Maillard reaction, Non-enzymatic glycosylation

Prof. Dr. Ralf Hoffmann
contact affiliation	Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, and Center for Biotechnology and Biomedicine, Universität Leipzig, Leipzig, Germany
contact email	hoffmann@chemie.uni-leipzig.de
lab head
Sanja Milkovska-Stamenova
contact affiliation	Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, and Center for Biotechnology and Biomedicine, Universität Leipzig, Leipzig, Germany
contact email	sanja.milkovska@uni-leipzig.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003054
     1. Label: PRIDE project
     2. Name: Bovine protein glycation sites in different milk products