PXD003029-1

PXD003029 is an original dataset announced via ProteomeXchange.

Title	CD147 mutants affinity purification mass spectrometry from human T cells
Description	CD147 is known as tumor and regulatory T cell marker and as regulator of chemotaxis, stress response or immune cell function; however, the mode of action of CD147 remains incompletely understood. Using affinity purification mass spectrometry (AP-MS) of HA-tagged CD147 swap and deletion mutants, we examined the CD147 microenvironment. We found previously published interaction partners of CD147 and also identified two highly significant new ones: the plasma membrane calcium ATPase 4 (PMCA4) and moesin. The use of CD147 mutants allowed determining of the respective CD147 parts essential for interaction. Finally, we performed AP-MS of endogenous CD147 from primary human CD4+ T cells and again found CD147 associated with PMCA4.
HostingRepository	PRIDE
AnnounceDate	2016-01-28
AnnouncementXML	Submission_2016-01-28_00:09:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD003029
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Goran Mitulović
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss: -17.026549; Oxidation: 15.994915; Phospho: 79.966331; Carbamidomethyl: 57.021464; Acetyl: 42.010565; Glu->pyro-Glu: -18.010565; Gln->pyro-Glu: -17.026549
Instrument	LTQ Velos; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-11 23:51:04	ID requested
⏵ 1	2016-01-28 00:09:27	announced

Supper V, Schiller HB, Paster W, Forster F, Boul, è, gue C, Mitulovic G, Leksa V, Ohradanova-Repic A, Machacek C, Schatzlmaier P, Zlabinger GJ, Stockinger H, Association of CD147 and Calcium Exporter PMCA4 Uncouples IL-2 Expression from Early TCR Signaling. J Immunol, 196(3):1387-99(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Human, Jurkat, primary T cells, LC-MS/MS, CD147, affinity purification

Verena Supper
contact affiliation	Medizinische Universität Wien Center for Pathophysiology, Infectiology and Immunology Lazarettgasse 19 1090 Vienna, Austria
contact email	verena.supper@meduniwien.ac.at
lab head
Goran Mitulović
contact affiliation	Medizinische Universität Wien
contact email	goran.mitulovic@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD003029

PRIDE project URI

• PRIDE
  1. PXD003029
     1. Label: PRIDE project
     2. Name: CD147 mutants affinity purification mass spectrometry from human T cells