PXD002839-1

PXD002839 is an original dataset announced via ProteomeXchange.

Title	Nuclear phosphoproteomics analysis of resting and IL-2-stimulated T lymphocytes
Description	The aim of the present project was to study in detail the site-specific phosphorylation events occurring both in resting as well as in T lymphocytes stimulated with IL-2 for five minutes. For that purpose we combined SILAC-based quantitative mass spectrometry analysis with phosphopeptide enrichment using TiO2 beads. We performed three biological replicas of the same experiment which resulted in the identification of above 8500 unique phosphosites corresponding to more than 3000 proteins. From the 6145 phosphosites that were consistently quantified in at least 2 out of the 3 replicas performed, we detected that 390 were regulated by IL-2 being the up-regulated phosphosites five times more abundant than the down-regulated ones. Those IL-2-dependent phosphosites corresponded to distinct proteins involved in distinct aspects of gene expression and cell cycle regulation.
HostingRepository	PRIDE
AnnounceDate	2016-04-18
AnnouncementXML	Submission_2016-04-18_07:31:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nerea Osinalde
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-02 01:54:27	ID requested
⏵ 1	2016-04-18 07:31:57	announced

Osinalde N, Mitxelena J, S, á, nchez-Quiles V, Akimov V, Aloria K, Arizmendi JM, Zubiaga AM, Blagoev B, Kratchmarova I, Nuclear Phosphoproteomic Screen Uncovers ACLY as Mediator of IL-2-induced Proliferation of CD4+ T lymphocytes. Mol Cell Proteomics, 15(6):2076-92(2016) [pubmed]

curator keyword: Biological

submitter keyword: Phosphorylation, IL-2, T-lymphocyte, nucleus

Irina Kratchmarova
contact affiliation	Associate Professor Department of Biochemistry and Molecular Biology University of Southern Denmark Campusvej 55, 5230 Odense M Denmark e-mail: ihk@bmb.sdu.dk Phone: (+45) 6550 2494 Fax: (+45) 6593 3018 http://www.cebi.sdu.dk
contact email	ihk@bmb.sdu.dk
lab head
Nerea Osinalde
contact affiliation	Postdoc
contact email	nmo@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD002839

PRIDE project URI

• PRIDE
  1. PXD002839
     1. Label: PRIDE project
     2. Name: Nuclear phosphoproteomics analysis of resting and IL-2-stimulated T lymphocytes