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PXD002791-1

PXD002791 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUbiquitin-activated interaction traps (UBAITs) identify E3 ligase binding partners
DescriptionWe describe a new class of reagents for identifying substrates, adaptors, and regulators of HECT and RING E3s. UBAITs (Ubiquitin-Activated Interaction Traps) are E3-ubiquitin fusion proteins, and in an E1- and E2-dependent manner, the C-terminal ubiquitin moiety forms an amide linkage to proteins that interact with the E3, enabling covalent co-purification of the E3 with partner proteins. UBAITs were made for both HECT (Rsp5, Itch) and RING (Psh1, RNF126, RNF168) E3s. For HECT E3s, substrate trapping occurred in vitro either through an E3 thioester-linked lariat intermediate or an E2 thioester intermediate, and both WT and active site mutant UBAITs trapped known interacting proteins in yeast and human cells. Yeast Psh1 and human RNF126 and RNF168 UBAITs also trapped known interacting proteins when expressed in cells.
HostingRepositoryMassIVE
AnnounceDate2015-08-25
AnnouncementXMLSubmission_2015-08-25_08:55:09.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndre Bui
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationListubiquitination signature dipeptidyl lysine; Carbamidomethyl; Oxidation
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-25 08:35:33ID requested
12015-08-25 08:55:09announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: UBAITs, ubiquitin, ubiquitin ligases, human 293T, Saccharomyces cerevisiae
Contact List
Jon Huibregtse
lab head
Andre Bui
contact affiliationUniversity of Texas at Austin
contact emailpmaf@austin.utexas.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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