PXD002448-1

PXD002448 is an original dataset announced via ProteomeXchange.

Title	Composition of Rosenthal fibers in Alexander disease patients and mouse models
Description	Alexander disease (AxD) is a fatal neurodegenerative disorder characterized by the presence in astrocytes of protein aggregates called Rosenthal fibers (RFs). In this work, we used a new biochemical fractionation method to enrich for RFs followed by analysis of this fraction using quantitative iTRAQ mass spectrometry. This work identified 77 proteins not previously associated with RFs. Multiple proteins selected for follow-up were confirmed by immunobloting to be enriched in the RF fraction of both the AxD mouse models and human AxD patients including receptor for activated protein C kinase 1 (RACK1), G1/S-specific cyclin D2, and ATP-dependent RNA helicase DDX3X.
HostingRepository	PRIDE
AnnounceDate	2016-05-23
AnnouncementXML	Submission_2016-05-23_00:50:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Brenner
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-30 01:58:56	ID requested
⏵ 1	2016-05-23 00:50:54	announced

Heaven MR, Flint D, Randall SM, Sosunov AA, Wilson L, Barnes S, Goldman JE, Muddiman DC, Brenner M, Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease. J Proteome Res, 15(7):2265-82(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: iTRAQ 8-plex, Alexander disease, Rosenthal fibers

Michael Brenner
contact affiliation	Professor of Neurobiology, University of Alabama at Birmingham, USA
contact email	michaelb@uab.edu
lab head
Michael Brenner
contact affiliation	University of Alabama at Birmingham
contact email	michaelb@uab.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002448
     1. Label: PRIDE project
     2. Name: Composition of Rosenthal fibers in Alexander disease patients and mouse models