PXD002349 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | On the relationship of protein and mRNA dynamics in vertebrate embryonic development |
Description | How the fertilized egg develops the anatomy of an adult ultimately must be explained at the biochemical level. Deeply connected to the anatomy is the complement of proteins and RNAs expressed during embryogenesis. The relationship of mRNA and respective protein expression dynamics during development has never been systematically explored. Here we present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus, between unfertilized egg and early tadpole stage. Surprisingly, most proteins change very little; there is an inverse correlation between abundance and rate of change. While the correlation of mRNA and protein expression is poor, a simple mass action kinetics model connects protein to RNA. Parameterized by two fixed parameters the median protein synthesis and degradation rates, the model depends on RNA dynamics, and initial protein concentration. Associated mRNA datasets in GEO: GSE73905 and GSE73870. |
HostingRepository | PRIDE |
AnnounceDate | 2015-11-17 |
AnnouncementXML | Submission_2015-11-17_00:30:52.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Wuehr |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-06-11 01:49:28 | ID requested | |
1 | 2015-10-20 02:56:10 | announced | |
2 | 2015-10-20 03:14:34 | announced | Updated publication details. |
3 | 2015-11-16 05:59:47 | announced | Updated publication details. |
⏵ 4 | 2015-11-17 00:30:55 | announced | Updated project metadata. |
Publication List
Peshkin L, W, ΓΌ, hr M, Pearl E, Haas W, Freeman RM, Gerhart JC, Klein AM, Horb M, Gygi SP, Kirschner MW, On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development. Dev Cell, 35(3):383-94(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: mRNA dynamics, protein dynamics, embryonic development, Xenopus |
Contact List
Steven Gygi |
contact affiliation | Harvard Medical School |
contact email | steven_gygi@hms.harvard.edu |
lab head | |
Martin Wuehr |
contact affiliation | Harvard Medical School |
contact email | martin.wuehr@gmx.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002349
- Label: PRIDE project
- Name: On the relationship of protein and mRNA dynamics in vertebrate embryonic development