PXD002349

PXD002349 is an original dataset announced via ProteomeXchange.

Title	On the relationship of protein and mRNA dynamics in vertebrate embryonic development
Description	How the fertilized egg develops the anatomy of an adult ultimately must be explained at the biochemical level. Deeply connected to the anatomy is the complement of proteins and RNAs expressed during embryogenesis. The relationship of mRNA and respective protein expression dynamics during development has never been systematically explored. Here we present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus, between unfertilized egg and early tadpole stage. Surprisingly, most proteins change very little; there is an inverse correlation between abundance and rate of change. While the correlation of mRNA and protein expression is poor, a simple mass action kinetics model connects protein to RNA. Parameterized by two fixed parameters the median protein synthesis and degradation rates, the model depends on RNA dynamics, and initial protein concentration. Associated mRNA datasets in GEO: GSE73905 and GSE73870.
HostingRepository	PRIDE
AnnounceDate	2015-11-17
AnnouncementXML	Submission_2015-11-17_00:30:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Wuehr
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-11 01:49:28	ID requested
1	2015-10-20 02:56:10	announced
2	2015-10-20 03:14:34	announced	Updated publication details.
3	2015-11-16 05:59:47	announced	Updated publication details.
⏵ 4	2015-11-17 00:30:55	announced	Updated project metadata.

Peshkin L, W, ü, hr M, Pearl E, Haas W, Freeman RM, Gerhart JC, Klein AM, Horb M, Gygi SP, Kirschner MW, On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development. Dev Cell, 35(3):383-94(2015) [pubmed]

curator keyword: Biological

submitter keyword: mRNA dynamics, protein dynamics, embryonic development, Xenopus

Steven Gygi
contact affiliation	Harvard Medical School
contact email	steven_gygi@hms.harvard.edu
lab head
Martin Wuehr
contact affiliation	Harvard Medical School
contact email	martin.wuehr@gmx.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD002349

PRIDE project URI

• PRIDE
  1. PXD002349
     1. Label: PRIDE project
     2. Name: On the relationship of protein and mRNA dynamics in vertebrate embryonic development