PXD002239-1

PXD002239 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Network Analysis in the Sea Urchin Strongylocentrotus purpuratus Reveals New Candidates in Egg Activation
Description	Fertilization triggers a dynamic symphony of molecular transformations induced by a rapid rise in intracellular calcium. Most prominent are surface alterations, metabolic activation, cytoskeletal reorganization, and cell cycle reentry. While the activation process appears to be broadly evolutionarily conserved, and protein phosphorylation is known to play a key role, the signaling networks mediating the response to fertilization are not well described. To address this gap, we performed a time course phosphoproteomic analysis of egg activation in the sea urchin Strongylocentrotus purpuratus, a system that offers biochemical tractability coupled with exquisite synchronicity. By coupling large-scale phosphopeptide enrichment with unbiased quantitative mass spectrometry, we identified striking changes in global phosphoprotein patterns at 2- and 5- min post fertilization as compared to unfertilized eggs. Overall, we mapped 8796 distinct phosphosite modifications on 2833 phosphoproteins, of which 15% were differentially regulated in early egg activation. Activated kinases were identified by phosphosite mapping, while enrichment analyses revealed conserved signaling cascades not previously associated with egg activation. This work represents the most comprehensive study of signaling associated with egg activation to date, providing both novel mechanistic insights and a valuable resource for the broader research community.
HostingRepository	PRIDE
AnnounceDate	2015-08-04
AnnouncementXML	Submission_2015-08-04_12:45:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongbo Guo
SpeciesList	scientific name: Strongylocentrotus purpuratus (Purple sea urchin); NCBI TaxID: 7668;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-27 07:46:36	ID requested
⏵ 1	2015-08-04 12:45:33	announced

Guo H, Garcia-Vedrenne AE, Isserlin R, Lugowski A, Morada A, Sun A, Miao Y, Kuzmanov U, Wan C, Ma H, Foltz K, Emili A, Phosphoproteomic network analysis in the sea urchin Strongylocentrotus purpuratus reveals new candidates in egg activation. Proteomics, 15(23-24):4080-95(2015) [pubmed]

curator keyword: Biological

submitter keyword: calcium / egg activation / network analysis / phosphoproteomics

Andrew Emili
contact affiliation	University of Toronto
contact email	andrew.emili@utoronto.ca
lab head
Hongbo Guo
contact affiliation	University of Toronto
contact email	hongboguo88@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002239
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Network Analysis in the Sea Urchin Strongylocentrotus purpuratus Reveals New Candidates in Egg Activation