PXD002209 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | iTRAQ-Facilitated Proteomic Profiling of Cotton Anthers |
Description | Cotton (Gossypium hirsutum L.) is an important economic crop, used mainly for the production of textile fiber. Using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant CCRI9106 was isolated from the wild-type upland cotton cultivar CCRI040029. To use CCRI9106 in cotton hybrid breeding, it is of great importance to study the molecular mechanisms of its male sterility. Here, histological and iTRAQ-facilitated proteomic analyses of anthers were performed to explore male sterility mechanisms of the mutant. Scanning and transmission electron microscopy of the anthers showed that the development of pollen wall in CCRI9106 was severely defective with a lack of exine formation. At the protein level, 6,121 high-confidence proteins were identified and 365 of them showed differential expression patterns between mutant and wild-type anthers. The proteins up- or down-regulated in MT anthers were mainly involved in exine formation, protein degradation, calcium ion binding and etc. These findings provide valuable information on the proteins involved in anther and pollen development, and contribute to elucidate the mechanism of male sterility in upland cotton. |
HostingRepository | PRIDE |
AnnounceDate | 2015-06-11 |
AnnouncementXML | Submission_2015-06-11_03:10:57.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ji Liu |
SpeciesList | scientific name: Gossypium hirsutum (Upland cotton) (Gossypium mexicanum); NCBI TaxID: 3635; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-05-20 02:59:58 | ID requested | |
⏵ 1 | 2015-06-11 03:10:58 | announced | |
Publication List
Liu J, Pang C, Wei H, Song M, Meng Y, Ma J, Fan S, Yu S, iTRAQ-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (Gossypium hirsutum L.). J Proteomics, 126():68-81(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: iTRAQ, Cotton, Anther |
Contact List
Chaoyou Pang |
contact affiliation | State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China |
contact email | liujicricaas@163.com |
lab head | |
Ji Liu |
contact affiliation | Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China. |
contact email | liujicricaas@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002209
- Label: PRIDE project
- Name: iTRAQ-Facilitated Proteomic Profiling of Cotton Anthers