PXD002209

PXD002209 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-Facilitated Proteomic Profiling of Cotton Anthers
Description	Cotton (Gossypium hirsutum L.) is an important economic crop, used mainly for the production of textile fiber. Using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant CCRI9106 was isolated from the wild-type upland cotton cultivar CCRI040029. To use CCRI9106 in cotton hybrid breeding, it is of great importance to study the molecular mechanisms of its male sterility. Here, histological and iTRAQ-facilitated proteomic analyses of anthers were performed to explore male sterility mechanisms of the mutant. Scanning and transmission electron microscopy of the anthers showed that the development of pollen wall in CCRI9106 was severely defective with a lack of exine formation. At the protein level, 6,121 high-confidence proteins were identified and 365 of them showed differential expression patterns between mutant and wild-type anthers. The proteins up- or down-regulated in MT anthers were mainly involved in exine formation, protein degradation, calcium ion binding and etc. These findings provide valuable information on the proteins involved in anther and pollen development, and contribute to elucidate the mechanism of male sterility in upland cotton.
HostingRepository	PRIDE
AnnounceDate	2015-06-11
AnnouncementXML	Submission_2015-06-11_03:10:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ji Liu
SpeciesList	scientific name: Gossypium hirsutum (Upland cotton) (Gossypium mexicanum); NCBI TaxID: 3635;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-20 02:59:58	ID requested
⏵ 1	2015-06-11 03:10:58	announced

Liu J, Pang C, Wei H, Song M, Meng Y, Ma J, Fan S, Yu S, iTRAQ-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (Gossypium hirsutum L.). J Proteomics, 126():68-81(2015) [pubmed]

curator keyword: Biological

submitter keyword: iTRAQ, Cotton, Anther

Chaoyou Pang
contact affiliation	State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China
contact email	liujicricaas@163.com
lab head
Ji Liu
contact affiliation	Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan Province, China.
contact email	liujicricaas@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD002209

PRIDE project URI

• PRIDE
  1. PXD002209
     1. Label: PRIDE project
     2. Name: iTRAQ-Facilitated Proteomic Profiling of Cotton Anthers