PXD002192

PXD002192 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of cysteine cathepsin substrates shed from the surface of cancer cells
Description	Extracellular cysteine cathepsins are known to drive cancer progression, but besides degradation of extracellular matrix proteins little is known about their physiological substrates and thus the molecular mechanisms they deploy. One of the major mechanisms used by other extracellular proteases to facilitate cancer progression is proteolytic release of the extracellular domains of transmembrane proteins or ectodomain shedding. Here we show using a mass spectrometry-based approach that cathepsins L and S act as sheddases and cleave extracellular domains of CAM adhesion proteins and transmembrane receptors from the surface of cancer cells.
HostingRepository	PRIDE
AnnounceDate	2015-06-23
AnnouncementXML	Submission_2015-06-23_04:19:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Fonovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; acetate labeling reagent (N-term) (heavy form: +3amu)
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-18 04:08:20	ID requested
⏵ 1	2015-06-23 04:19:44	announced

Soboti, č B, Vizovi, š, ek M, Vidmar R, Van Damme P, Gocheva V, Joyce JA, Gevaert K, Turk V, Turk B, Fonovi, ć M, Proteomic Identification of Cysteine Cathepsin Substrates Shed from the Surface of Cancer Cells. Mol Cell Proteomics, 14(8):2213-28(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Degradomics, proteases, cathepsins, cancer, shedding, substrates

Marko Fonovic
contact affiliation	Department of Biochemistry and Molecular and Structural Biology Jozef Stefan Institute Slovenia
contact email	marko.fonovic@ijs.si
lab head
Marko Fonovic
contact affiliation	Jozef Stefan Institute
contact email	marko.fonovic@ijs.si
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD002192

PRIDE project URI

• PRIDE
  1. PXD002192
     1. Label: PRIDE project
     2. Name: Proteomic identification of cysteine cathepsin substrates shed from the surface of cancer cells