PXD002183 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of Trophoblast Stem Cells |
Description | Esrrb is a transcription factor implicated in embryonic stem (ES) cell self-renewal, yet its knockout causes intrauterine lethality due to defects in trophoblast development. Here we show that in trophoblast stem (TS) cells, Esrrb is a downstream target of fibroblast growth factor (Fgf) signalling and is critical to drive TS cell self-renewal. In contrast to its occupancy of pluripotency-associated loci in ES cells, Esrrb sustains the stemness of TS cells by direct binding and regulation of TS cell-specific transcription factors including Elf5 and Eomes. To elucidate the mechanisms whereby Esrrb controls the expression of its targets, we characterized its TS cell-specific interactome by mass spectrometry. Unlike in ES cells, Esrrb interacts in TS cells with the histone demethylase Lsd1 and with the RNA Polymerase II-associated Integrator complex. Our findings provide new insights into both, the general and context-dependent wiring of transcription factor networks in stem cells by master transcription factors. |
HostingRepository | PRIDE |
AnnounceDate | 2015-07-24 |
AnnouncementXML | Submission_2015-07-27_04:59:08.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Oxley |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-05-13 08:00:26 | ID requested | |
1 | 2015-07-24 00:50:13 | announced | |
⏵ 2 | 2015-07-27 04:59:09 | announced | Updated publication reference for PubMed record(s): 26206133. |
Publication List
Latos PA, Goncalves A, Oxley D, Mohammed H, Turro E, Hemberger M, Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells. Nat Commun, 6():7776(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: TS cells, self-renewal, transcription factors, Esrrb, LC-MS/MS |
Contact List
david oxley |
contact affiliation | Babraham Institute Cambridge UK |
contact email | david.oxley@babraham.ac.uk |
lab head | |
David Oxley |
contact affiliation | Babraham Institute |
contact email | david.oxley@babraham.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002183
- Label: PRIDE project
- Name: Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of Trophoblast Stem Cells