PXD002064-1

PXD002064 is an original dataset announced via ProteomeXchange.

Title	Sinorhizobium meliloti Ser/Thr/Tyr phosphoproteome
Description	Sinorhizobium meliloti, a facultative microsymbiont of alfalfa, should fine-tune its cellular processes to live saprophytically in soils characterized with limited nutrients and diverse stresses. In this study, TiO2 enrichment and LC-MS/MS were used to uncover the site-specific Ser/Thr/Tyr phosphoproteome of S. meliloti in minimum medium at stationary phase. There are a total of 96 unique phosphorylated sites, with a Ser/Thr/Tyr distribution of 65:32:5, in 78 proteins. Phosphoproteins identified in S. meliloti showed a wide distribution pattern regarding to functional categories, such as replication, transcription, translation, posttranslational modification, transport and metabolism of amino acids, carbohydrate, inorganic ion, succinoglycan etc. Ser/Thr/Tyr phosphosites identified within the conserved motif in proteins of key cellular function indicate a crucial role of phosphorylation in modulating cellular physiology. Moreover, phosphorylation events potentially involved in rhizobial adaptation to diverse stresses were also discussed, such as those identified in SMa0114 and PhaP2 (polyhydroxybutyrate synthesis), ActR (pH stress and microaerobic adaption), SupA (potassium stress), chaperonin GroEL2 (viability and potentially symbiosis), and ExoP (succinoglycan synthesis and secretion). These Ser/Thr/Tyr phosphosites identified herein would be helpful for our further investigation and understanding of the role of phosphorylation in rhizobial physiology.
HostingRepository	PRIDE
AnnounceDate	2015-10-05
AnnouncementXML	Submission_2015-10-05_06:05:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chang Fu Tian
SpeciesList	scientific name: Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti); NCBI TaxID: 382;
ModificationList	phosphorylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-14 01:31:33	ID requested
⏵ 1	2015-10-05 06:05:27	announced

Liu T, Tian CF, Chen WX, Site-Specific Ser/Thr/Tyr Phosphoproteome of Sinorhizobium meliloti at Stationary Phase. PLoS One, 10(9):e0139143(2015) [pubmed]

curator keyword: Biological

submitter keyword: phosphorylation, sinorhizobium meliloti

Chang Fu TIAN
contact affiliation	State Key Laboratory of Agrobiotechnology, and College of Biological Sciences, China Agricultural University, Beijing, China
contact email	cftian@cau.edu.cn
lab head
Chang Fu Tian
contact affiliation	China Agricultural University
contact email	cftian@cau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002064
     1. Label: PRIDE project
     2. Name: Sinorhizobium meliloti Ser/Thr/Tyr phosphoproteome