PXD002054

PXD002054 is an original dataset announced via ProteomeXchange.

Title	Bank Vole Protein Turnover Study C4PR_LIV
Description	We have used dietary administration of stable isotope labelled lysine to assess protein turnover rates for proteins from four tissues in the bank vole, Myodes glareolus. The annotated genome for this species is not available, so protein identification was attained through cross-species matching to the mouse. For proteins for which confident identifications were derived, the pattern of lysine incorporation over 40d was used to define the rate of synthesis of individual proteins in the four tissues. The data were heavily filtered to retain a very high quality data-set of turnover rates for 1088 proteins. Comparative analysis of the four tissues revealed different median rates of degradation (kidney: 0.099 per day; liver 0.136 per day; heart, 0.054 per day and skeletal muscle, 0.035 per day). These data were compared with protein degradation rates from other studies on intact animals or from cells in culture.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_00:49:55.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002054
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dean Hammond
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Myodes glareolus (Bank vole) (Clethrionomys glareolus); NCBI TaxID: 447135;
ModificationList	Oxidation; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-13 01:55:40	ID requested
1	2016-02-09 08:26:16	announced
2	2017-02-16 00:30:26	announced	Updated project metadata.
⏵ 3	2017-10-24 00:49:56	announced	Updated project metadata.

Hammond DE, Claydon AJ, Simpson DM, Edward D, Stockley P, Hurst JL, Beynon RJ, Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals. Mol Cell Proteomics, 15(4):1204-19(2016) [pubmed]

submitter keyword: Vole Protein Turnover

Robert John Beynon
contact affiliation	University of Liverpool
contact email	rbeynon@liverpool.ac.uk
lab head
Dean Hammond
contact affiliation	University of Liverpool
contact email	d.e.hammond@liv.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002054

PRIDE project URI

• PRIDE
  1. PXD002054
     1. Label: PRIDE project
     2. Name: Bank Vole Protein Turnover Study C4PR_LIV